Composite

Part:BBa_K5048074:Design

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-24)


araBAD promoter-SUMO-HSP60-FLAG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 582
    Illegal PstI site found at 371
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 582
    Illegal PstI site found at 371
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 582
    Illegal BglII site found at 323
    Illegal BglII site found at 471
    Illegal BamHI site found at 239
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 582
    Illegal PstI site found at 371
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 582
    Illegal PstI site found at 371
    Illegal NgoMIV site found at 986
    Illegal AgeI site found at 74
    Illegal AgeI site found at 1141
    Illegal AgeI site found at 1619
    Illegal AgeI site found at 2421
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56


Design Notes

We used homologous recombination to construct this part, so we need to design proper homologous arms. However, when we encounter the situation that two segments come from the same kind of backbone, we need to pay extra attention to the specificity of the primers.


Source

See our parts:BBa_K5048016, BBa_K5048065,BBa_K5048064,BBa_B0015


References